Modern methods in analysis of microtubules dynamic in vivo

Abstract

Microtubules are involved in any vital cellular activities, including the maintenance of cell shape, division, migration and intracellular transport. Microtubule dynamics is regulated by the balance between their polymerization and depolymerization. Microtubule stability is dependent on their alpha and beta subunits composition, tubulin post-translational modifications and interaction of microtubules with microtubule-associated proteins(MAPs). Disruption of these processes can lead to a number of pathological conditions such as cancer, cardiovascular disease, or the fibrosis development. This review summarizes the current knowledge of the modern methods of microtubule polymerization analysis. This allows a better understanding of the structure and mechanisms played by microtubules in their physiological functions and the development of pathological conditions resulting from their disorder.