HRM-PCR in medical diagnostic

Authors

  • Damian Nikodem Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland
  • Tomasz Cłapa Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland
  • Dorota Narożna Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland

DOI:

https://doi.org/10.18388/pb.2021_373

Abstract

High resolution melting (HRM) is a method based on the identification of differences in the denaturation of PCR reaction products in the presence of fluorescent dyes. It allows for a detailed analysis of the genetic profiles. In addition this analysis is low-cost, single-step, closed-tube and has high sensitivity. HRM found applications in diagnostics, laboratory and clinical researches. This article is a literature review of the applications of HRM analysis in medicine.

Downloads

Download data is not yet available.

Downloads

Published

2021-03-04

Issue

Vol. 67 No. 1 (2021)

Section

Articles

License

Copyright (c) 2021 Advances in Biochemistry

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

All journal contents are distributed under the Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0) license. Everybody may use the content following terms: Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made, ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original. There are no additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.

Copyright for all published papers © stays with the authors.

Copyright for the journal: © Polish Biochemical Society.