HRM-PCR in medical diagnostic

Authors

  • Damian Nikodem Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland
  • Tomasz Cłapa Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland
  • Dorota Narożna Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632 Poznań, Poland

DOI:

https://doi.org/10.18388/pb.2021_373

Abstract

High resolution melting (HRM) is a method based on the identification of differences in the denaturation of PCR reaction products in the presence of fluorescent dyes. It allows for a detailed analysis of the genetic profiles. In addition this analysis is low-cost, single-step, closed-tube and has high sensitivity. HRM found applications in diagnostics, laboratory and clinical researches. This article is a literature review of the applications of HRM analysis in medicine.

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Published

2021-03-04

Issue

Vol. 67 No. 1 (2021)

Section

Articles

License

Copyright (c) 2021 Advances in Biochemistry

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